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However, there is substantial evidence that ETAA1 requires phosphorylation for robust activation of ATR, with the individual S95D and S111D phosphomimetic mutations potentiating ATR activation by ectopically expressed ETAA1 AAD , and the double mutant acting synergistically 20

(2003) demonstrated the importance of time-specific efflux pump functionality showing that the highest levels of transcription and, therefore, efflux pump expression occurred early in biofilm development, and that this was a coordinated response of several efflux pumps causing increased levels of resistance