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Forebrain sections were blocked with 10% goat serum (1 h), incubated (4 C, overnight) with primary antibodies, followed by Cy3-conjugated Goat Anti-Rabbit IgG (1 h)

To measure the percentages of CB1 colocalization in VGAT- and VGLUT1-immunoreactive areas of DG, an unbiased physical dissector-based counting of CB1/VGAT or CB1/VGLUT1 double immunoreactive puncta was performed following the protocols described in Bolte and Cordelires [30]

Product identity is the one practical point worth emphasizing, because it is where the biggest gap between the trial and a vial opens up

To add further advantages, a number of tools have been provided in the database to facilitate the extraction and analysis of the compiled information

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