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Membranes were incubated with primary antibodies at 4 C overnight, followed by incubation with alkaline phosphatase (AP)-conjugated secondary antibodies (CST, USA) for 4 h at room temperature

Thus MtGLYI -8, MtGLYI -21, and MtGLYII -9 found to be the highly up-regulated abiotic stress responsive member that could be characterized further

Finally, the two researchers cross-checked the screening results

This process mimics natural fat digestion and allows the body to achieve high bioavailability of active substances

Injection frequency matters more than total weekly dose