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Chromatographic separation of AAs was achieved by applying 3 l of dissolved sample on a SeQuant ZIC-HILIC column (3.5 m particles, 100 2.1 mm) (Merck), combined with a Javelin particle filter (Thermo Fisher Scientific), and a SeQuant ZIC-HILIC precolumn (5 m particles, 20 2 mm) (Merck) using a linear gradient of mobile phase A (5 mM NH 4 OAc in acetonitrile/H 2 O (5/95, vol/vol)) and mobile phase B (5 mM NH4OAc in acetonitrile/H 2 O (95/5, vol/vol))

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To establish CGA stably transfected cells, CGA cDNA was subcloned into a pLVX-IRES-Puro vector

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Milk from grass-fed cows has higher levels of conjugated linoleic acids (CLA) and contains a proper balance of essential fatty acids both which have favorable outcomes on metabolism, lean body mass, brain health, and cancer prevention

It can also promote the production of interferon and increase T cell activation, while raising cAMP levels in tumor cells, thereby inhibiting their proliferation