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Analysis of 100,000 human cancer genomes reveals the landscape of tumor mutational burden

Detection of mitochondrial ROS, apoptosis, and viability For mitochondrial ROS measurements, cells were stained with MitoSOX Red (2.5 M, 30 minutes, dark), washed with phosphate-buffered saline (PBS), detached using trypsin-EDTA, and washed twice in Hanks Balanced Salt Solution (HBSS) by centrifugation (5 min, 1200 rpm, room temperature)

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Flavonoids are important antioxidants, and the Apiaceae family, including fennel, has many

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