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For generation of empty vector (pBABEneo), pBABEneo-HRASV12 was digested with BamHI and EcoRI to excise the HRASV12 insert

Although inflammaging and impaired proteostasis are both hallmarks of aging HSCs, a link between them remains unexplored

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An organismal aging model using the 3907 organ-nonspecific proteins and a conventional aging model using all 4778 QCed proteins on the SomaScan assay were also trained

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Our findings may suggest a synergistic effect between ALC therapy and an absence of concomitant drug treatments, potentially due to reduced pharmacological interference or enhanced metabolic responses to the therapy