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The SPR and recombinant-protein assays provide stronger evidence for a direct interaction than the earlier pathway studies

In such structural configuration, the two c-METs are placed in relatively close proximity, which enables their intracellular KDs to undergo autophosphorylation and thus initializing the downstream signaling (Fig

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In contrast, the bulkier Y37 rAmy in rAmy-bound AMY 2 R prevents equivalent interactions of N35 rAmy with RAMP2, and instead the bulkier Y37 rAmy forms interactions with E101 RAMP2 and F111 RAMP2 stabilising the peptide at the CTR/RAMP ECD interface in the bypass conformation