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10.1073/pnas.222546799 57 FremeauR

Upon elution of the supermix bound fraction, both columns were neutralized and re-equilibrated by flowing 100% Buffer C (1X Seppro Neutralization Buffer, Sigma) in tandem over both columns at 2 mL/min for 18 min, followed by re-equilibration with 100% Buffer A for 20 min

This ratio is in the range of 500

In locally advanced disease, such combinatorial approaches may theoretically enhance treatment responses in preclinical models, which could provide a conceptual basis for less invasive interventions