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7A, binding of plasminogen to all Chi proteins as well as HcpA of B

To the resin was added a solution of Fmoc-Sar-Sar-OH (3 eq), HATU (2.9 eq) and DIPEA (6 eq) in DMF (1 mL per 100 mg of resin)

Replacement of Asn450 by glutamine (N450Q), aspartate (N450D), and alanine (N450A) showed a significant increase in transpeptidase activity up to 3.6-fold enhancement and minor maturational defects, suggesting significant contribution of this residue in substrate binding and catalysis

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Proliferation index was determined by quantifying the fraction of Ki67-labelled cells/HNA-labelled cells using confocal microscopy at 20x magnification

in Political Science from the University of California San Diego