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All animal experiments were approved by the Institutional Animal Care and Use Committee of China Medical University and performed in accordance to the Guide for the Care and Use of Laboratory Animals (Ministry of Science and Technology of China, 2006) and the related ethical regulations of our university

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Glutathione also acts as a substrate in other detoxifying enzymes against oxidative stress, such as glutathione transferases

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The homogenates were then centrifuged (Eppendorf, Mumbai, India) at 10,000 rpm (15 min at 20 C), and the supernatants were collected