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(2014) 4:e979108

The reaction was initiated by adding 100 L of 2.5 mM nicotinamide adenine dinucleotide phosphate (NADPH), and after mixing thoroughly, the rate of absorption change at 412 nm was measured spectrophotometrically

2022;66:e2200196

Bacteriostatic water is the preferred reconstitution vehicle for peptides that will be stored and used over multiple days or weeks

All samples were analyzed immediately, or within the first 24 h, after being stored in a refrigerator below 5C to prevent degradation and microbial activity

Then, samples were centrifugated at 15,000 g for 10 min at 4 C, and the supernatant, containing the extracted D-/L-lactate, was moved into clean tubes