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La Cruz et al

1 Introduction Biologically active compounds are synthesized mostly by fungi, bacteria, archaea, and plants

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WADA and Athletic Bans MOTSc falls under WADAs Prohibited List , category S2 Classified alongside peptide hormones, growth factors, and related substances Banned in- and out-of-competition Positive tests may result in disqualification or suspension WADA prohibits all synthetic analogues of mitochondrial and peptide growth factors, including MOTS-c. World Anti-Doping Agency, Prohibited List Global Legal Overview Canada, UK, Australia : MOTSc is legal for research but not human use EU : Generally treated the same as RUO peptides

POLG is crucial for mtDNA replication by binding to the D-loop region and plays a key role in repairing mitochondrial DNA (mtDNA) damage (Facchinello et al., 2021)