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After centrifugation at 16,000 g for 10 min at 4 C, 150 l of the supernatant was collected for LCMS analysis

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133 Subsequently, neutrophils may cross the continuous basement membrane via releasing proteases, such as matrix metalloproteinases (MMPs)

Molecular dynamics simulations of GLRX and complexes Using the crystal structure of GLRX (PDB ID: 4RQR, Uniprot ID: P35754), we constructed models of GLRX wild type and mutants (C83S, C8S, and C79S) as well as the GLRX dimers for molecular dynamics (MD) simulations, using Protein Preparation Wizard (Pymol, Schrdinger, Inc.) and the relaxation strategy in our prior work 52

Clostridium butyricum protects the epithelial barrier by maintaining tight junction protein expression and regulating microflora in a murine model of dextran sodium sulfate-induced colitis