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After two PBS washes, the cells were treated with RNase A (Gibco, Grand Island, NY) for 30 min at 4 C to remove RNA and ensure only DNA is stained with propidium iodide (PI) (Sigma-Aldrich)

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307 In a clinical trial based on health subjects, a selective COX-2 inhibitor celecoxib effectively enhanced the insulin sensitivity as examined by an insulin tolerance test, whereas the COX-1 inhibitor acetylsalicylic acid (ASA) exhibited negligible influence

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