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Using the distance matrix, population structure was assessed by conducting a Principal Component Analysis (PCA) and constructing a neighbour-joining (NJ) tree using the R package ape (v5.7)

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Data from scRNA-seq and Western blot analyses of kidney tissues from mice with oxalate-induced AKI, along with observations of primary PTECs treated with CaOX, either alone or co-incubated with ferroptosis-specific inhibitors, demonstrated the occurrence of ferroptosis in renal tubular cells within the oxalate-induced AKI model