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To validate this interaction, GST pull-down assays were performed and confirmed direct binding of HMGB1/2 to SLIT2-N terminal (N) and SLIT2-C terminal (C), with K-Hcy modification enhancing HMGB1/2 and SLIT2-N interaction strength

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Towards a unifying, systems biology understanding of large-scale cellular death and destruction caused by poorly liganded iron: Parkinsons, Huntingtons, Alzheimers, prions, bactericides, chemical toxicology and others as examples

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