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After a second centrifugation step, the pellet was resuspended in 500 l measurement buffer and fluorescence was measured immediately for 10 min

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This positive regulatory loop is particularly relevant in the context of neuronal regeneration, where the BDNF signaling cascade promotes axonal growth, synaptic remodeling, and the recovery of neural circuits [43, 44]

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10.1038/cddiscovery.2016.89 Cell Death Discov