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In HpGGT, substitution of Try433 with Phe (Y433F) had no effect on the catalytic activity, but mutation with alanine (Y433A) resulted in a dramatic decrease in activity

Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2 +-ATPase

This suggests that an undiscovered lipase remains in the JCVI-Syn3A genome

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The beads were washed three times with 80% (v/v) ethanol and then resuspended in 50 L of 50 mM ammonium bicarbonate (dissolved in water), followed by overnight incubation with 0.5 g of trypsin

Proper technique and aftercare are essential for the best outcomes