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PMID: 28797793

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(1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase

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MTA1 -knockout or MTA1 -overexpressed cells were seeded into 384-well assay plates using a Multidrop TM Combi reagent dispenser (Thermo Fisher Scientific) at a density of 1,000 cells per well in 50 l of RPMI medium supplemented with 10% FBS, 2% L-glutamine, 1% penicillinstreptomycin and 0.1% amphotericin B