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RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

Summary of indications of glutathione, route of administration, and mechanism of action RESULTS : Efficacy of Glutathione Supplementation for Skin Lightening Oral glutathione: Oral glutathione supplements have demonstrated mixed results in clinical studies, prompting further research to explore the effects of varying dosages, treatment durations, and individual factors [11]

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