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Samples were mixed with Griess reagent in a ratio of 1:1, and nitric oxide level (nitrite and nitrate) was quantified from sodium nitrate standard curve measured at 550 nm 102

So, the expression of FUR gene or inhibition of furin or obstructions and/or spoiling to form MBCS for the interaction between S1/S2 complex and furin are now the new strategies of target for the therapeutic approaches