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Bar graph and error bars represent mean SD, n=3 distinct samples (in each of the following two conditions, BMDM +IFN and RAW 264.7 + LPS + IFN + 1400W, citrulline was not detected in one of the 3 samples, so two dots are shown)

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The animal studies (mostly in mice and rats) are where the dramatic lifespan extension numbers come from

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doi: 10.1002/adbi.202400180 79

Likewise, nicotine but not nicotinic acid is an agonist of the nicotinic receptors that respond to the neurotransmitter, acetylcholine