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Molecular and cellular mechanism of actions of AST has revealed its potential in preclinical and clinical studies in prevention and treatment of CVDs

Transfected cells are grown in 12-well trays in culture medium containing 2% FCS for 48 hours, the culture medium is discarded, then the attached cells are gently washed with PBS

C.NenadicZ.DoA

Compounds were resolved over mobile phase A (10 mM ammonium acetate in 88% water/12% acetonitrile (MeCN), pH 6.85) and mobile phase B (10 mM ammonium acetate in 90% MeCN, pH 6.85) with column temperature 30 C using flow rate 0.25 mL/min under gradient conditions: 100% B, 0.5 min

The depletion of NADPH directly leads to a decrease in GSH levels, inactivates GPx4, and prevents the clearance of intracellular LPO, eventually causing the accumulation of LPO