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Although small and moderately sized molecules equilibrate rapidly enough to support continuous monitoring, many large proteins and slowly transported biomarkers remain poorly represented in the ISF and therefore require direct blood-based quantification [39,40]

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The hydrogen bond distances were monitored/corrected in SHELX (CCP4i) 49 , and the output file was then read in Coot

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All samples were imaged using a Leica TCS SP5 confocal microscope with 405 nm, 488 nm, 543 nm or 633 nm laser lines, scanning each channel separately under image capture conditions that eliminated channel overlap

Eight of the 12 subjects were reported to have reactive ileal lymphoid hyperplasia, with 3 of them also having colonic lymphoid hyperplasia, and 1 subject had just colonic lymphoid hyperplasia alone