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After being blocked, the membranes were incubated with primary antibodies overnight at 4 C, including GPX4 (1:1 000, #DF6701, Affinity, USA), FTL (1:5 000, Cat# 10727-1-AP, Proteintech), PTGS2 (1:1 000, AF7003, Affinity, USA), and -actin (1:1 000, #4970, Cell Signaling Technology, Danvers, MA, USA), and were then incubated with secondary antibodies

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Furthermore, Hu et al (42) discovered that the downregulation of the GPX3 gene resulted in a decrease in the wound healing capacity and transmembrane migration rate of OC and colorectal cancer cells

Elevating your arm and applying ice can ease any discomfort

Hold the needle there for 10 seconds after injecting, then pull it out

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