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The elution was dialyzed overnight against LS Buffer (25 mM HEPES/NaOH pH 7.5, 100 mM NaCl, 5% glycerol, 1 mM DTT) before loading onto a HiTrap SP HP column (GE Healthcare) equilibrated with LS Buffer

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For researchers handling multiple peptide types simultaneously, our guides on general peptide reconstitution and mixing peptides with bacteriostatic water provide the universal technique that applies across all compounds

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