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Before staining, slides were thawed to room temperature for 30 min and then fixed in 10% neutral buffered formalin for 20 min, rinsed in double-distilled water, followed by rinsing in 60% isopropanol before being placed in working ORO-isopropanol stain (Rowley Biochemical, H-503-1B) for 5 min

Treated rats showed reduced activation of microglia and astrocytes in the substantia nigra and striatum, as evidenced by lower expression of glial markers compared to untreated 6-OHDA controls (56)

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