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Autophagy was determined by (1) detection of autophagic flux, autophagosome substrates SQSTM1 and the specific processing of the autophagy protein LC3 with or without a 50 M lysosomal protease inhibitor, chloroquine, (Sigma, C6628) by western blot analysis 31

That was exactly what was happening in my case not overwhelming or jolting in any way, but a gentle boost

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