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Kinase activity was determined by the addition of 25 M ATP, 3 Ci of [- 32 P]-ATP (SRP-301, Hartmann Analytic), and 100 M of an optimal Abl substrate sequence carrying an N -terminal Biotin (Biotin-GGEAIYAAPFKK-amide) in kinase assay buffer (20 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 ,1 mM DTT) for 15 min at room temperature in a final assay volume of 20 L

10.1016/j.endmts.2021.100080 25 SharmaG.HuC.StaquicinD

Free Radic Biol Med 99:436450

Chronic stress promotes palatable feeding, which reduces signs of stress: feedforward and feedback effects of chronic stress

USA 109 , 899904 (2012)

Tissue-local accumulation at injury sites is plausible but not extensively characterised