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Mobile phase A was 10 mM ammonium acetate (pH 10, adjusted with ammonium hydroxide) in water and mobile phase B was prepared with 10% A / 90% ACN

By Value 6.3.1.2

doi: 10.1038/s41579-020-0433-9 49 FangC.KimH.YanagisawaL.BennettW.SirvenM

Interestingly, the activity of Trx1 was fully restored after long-term kinetic measurements (data not shown), indicating that the initial inhibitory effect of preheated APR-246 is transient