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Redox lipid reprogramming commands susceptibility of macrophages and microglia to ferroptotic death

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For the singleplex reactions, 2 L DNA (with a DNA concentration of 0.55 to 127 ng/L) were added to 8 L PCR Mix, consisting of 5 L Master Mix (TaqPath Pro Amp Master Mix, Applied Biosystems), 0.5 L Assay Mix, and 2.5 L H 2 O

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Complemented by structural-guided biochemical analyses, our studies reveal the mechanism of substrate recognition, the alternating access of NET, and elucidate the mode of action of the four antidepressants