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To determine albumin secretion, supernatant from 24 h was collected and the amount of albumin was determined using a human-specific albumin ELISA kit (Assay Pro) following the manufacturers instructions on an ELISA plate reader (Tecan Spark 20M)

50 mg Green Tea Extract Per Serving

After the fixation step in 2.5% v /v PFA for 5 min at room temperature, samples were analyzed with a Guava EasyCyte flow cytometer (Millipore) equipped with the InCyte software (Millipore)

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