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3.7 Molecular dynamics validation of RAGE-AB4 binding Molecular dynamics simulations confirmed the stable binding of AB4 to the V-domain of RAGE, with key findings as follows: (1) Structural stability: Over a 100 ns simulation period, the root-mean-square deviation (RMSD) of the ligand (AB4) remained within 24 , while the RMSD of the RAGE-AB4 complex was maintained at 25 (Figure 8A)

Theres a piece of folklore floating around the peptide community that goes something like this: 8 weeks on, 4 weeks off

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Ni-NTA affinity chromatography is a protein purification technique that relies on His-tag binding