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All data has been deposited in the NCBI GEO Database under Accession Number: GSE193468

Following immunostaining, L3 central nervous system (CNS) or body walls were mounted in SlowFade Gold (Invitorgen) and fluorescent images were acquired on a LSM 980 (Carl Zeiss) using a 20 objective (for the CNS) or 40/63 objective (for the NMJ)

doi: 10.1093/jnci/19.3.451

doi:10.1053/jvet.2003.50070

To strengthen the assumption that only the cyclic version of G4CP2 is responsible for the observed phenotypes, we generated a G4CP2 variant (linear G4CP2), where a stop codon is inserted between the G4CP2 sequence and the Intein N element (Supplementary Figure S7A)

By increasing actin production, TB-500 enhances cell motility, allowing repair cells to migrate more efficiently to sites of injury