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Knockdown of glutamate-cysteine ligase by small hairpin RNA reveals that both catalytic and modulatory subunits are essential for the survival of primary neurons

Replication outside the originating group is a meaningful credibility marker in any preclinical literature

In particular, once an X chromosome is chosen for inactivation, extensive DNA methylation occurs on this chromosome, especially at CpG islands within promoter regions of genes, ensuring long-term gene silencing on the inactive X (Xi) chromosome

The majority of these adverse effects eventually go away without the need for medical intervention