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After 48 h, cells were washed with ice-cold PBS and lysed with 700 l of non-denaturing DISC lysis buffer (30 mM Tris-HCl (pH 7.4), 120 mM NaCl, 2 mM EDTA, 2 mM KCl, 1% Triton X-100, Roche complete protease inhibitor cocktail, 10 M PR619 and 1 mM NEM) on ice for 30 min

For instance, AMPK inhibits mTORC1 signaling through phosphorylation of TSC2, while simultaneously activating the deacetylase activity of sirtuin 1 (SIRT1), collectively regulating autophagy and energy metabolism

All the line scan images were obtained at location of three-disc diameter distance from the optic nerve disc, and the average thickness of the GCC (from the inner limiting membrane to the outer boundary of the inner plexiform layer) was measured by circular-scanning around the optic nerve disc 11,12,13,14,53,82

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