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The His-tag does not usually affect the bioactivity of the protein in question, and the purification procedure is simple

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18 This approach is particularly suitable when drugs with new mechanisms of action, especially first-in-class drugs, are needed or when the disease involves multiple biological pathways and may require multitarget interventions

Eur Urol 62(1):160165

Evaluation of the novel antichagasic activity of [1,2,3]Triazolo[1,5-a]pyridine derivatives