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Transwell chambers were assayed for cell invasion ability by applying 100 L of DMEM-diluted Matrigel gel (3:7 dilution) to the chambers to be checked and incubated at 37 C for 30 min to form a gel, then pre-treated MIA PaCa-2 and PANC-1 cells were resuspended with DMEM and spread in small chambers at 50000/well, and the lower chamber was placed in 20% FBS DMEM, incubated at 37C 5% CO 2 for 48 hours

Despite these advantages several limitations remain

To determine whether NRF2 can localize to the Ptgir gene, we first analyzed NRF2 chromatin immunoprecipitation sequencing (ChIPseq) data from Keap1 / mouse embryonic fibroblasts (MEFs) that display constitutive NRF2 activity 31

injections of 100 nmol kg 1 GLP-1MK-801 ( n = 8 mice and n = 4 cages), 300 nmol kg 1 GLP-1MK-801 ( n = 8 mice and n = 4 cages) or vehicle (isotonic saline, n = 7 mice and n = 4 cages) in comparison with vehicle-treated age-matched chow-fed C57BL/6 J mice ( n = 16 mice and n = 8-11 cages) for 14 days

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Yang H, Mu J, Chen L, Feng J, Hu J, Li L, Zhou JM, Zuo J (2015) S-nitrosylation positively regulates ascorbate peroxidase activity during plant stress responses