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Then, cells were lysed in PBS, 1% Triton X-100 plus protease inhibitor cocktail (Thermo Fisher, A32955)

& Bartholomew, B

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Immunofluorescence staining The primary antibodies used for immunofluorescence staining were Puf-A (monoclonal antibody), NPM1 (sc-5564, Santa Cruz, TX, USA), S6 (sc-74459, Santa Cruz), L5 (ab157099, Abcam), fibrillarin (sc-374022, Santa Cruz), and fibrillarin (sc-374022, Santa Cruz)