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However, the high cost and technological demands of next-generation sequencing limit its widespread application, especially in low-income countries where brucellosis is often endemic (150, 152, 153)

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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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Another strategy is utilizing PARP inhibitors, which resulted in rescuing NAD + levels and decreasing A aggregation in brain samples of AD flies, however, this study did not include any measurements of mitochondrial function 275