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In addition to producing the main methyl donor, methionine is also indirectly involved in the synthesis of polyamines, which are crucial for cell growth

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Yi D, Wang Q, Zhao Y, Song Y, You H, Wang J, et al

Seahorse flux analysis Assays were performed using an XFe96 Extracellular Flux Analyzer (Agilent)

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Preclinical studies have demonstrated that transplanting fecal microbiota from patients with MDD to rodents induced depression-like behaviors, including anhedonia and anxiety-like behaviors, and altered tryptophan metabolism, while microbiota transfer from healthy donors reversed these behavioral phenotypes (Kelly et al., 2016