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There is one well-documented report of a rash in a breast-fed infant that resolved when the mother stopped acetaminophen use and recurred when she resumed acetaminophen use

Dalton, TP (2002)

& Hunter, T

T., Wu, E., Cannavo, A., Koch, W

Subsequent work (see Reference 138 ) demonstrated that there is a large family of HECT-domain containing proteins that all probably act to promote ubiquitin transfer through a similar mechanism

After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice