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Zhao Y, Vanhoutte PM, Leung SWS (2015) Vascular nitric oxide: beyond eNOS

Membrane was blocked overnight in 5% BSA in TBST on a shaker at 4 C

The scaffold was soaked in an IGF-1 plasmid solution with a Lipofection reagent

Mesenteric and subcutaneous WAT hypertrophy developed slower and did not appear to reach a maximum within the period studied, with minimal inflammation

Second, the search was expanded across three major academic databases: PubMed, Google Scholar, and Web of Science, ensuring broad coverage of both clinical and preclinical research