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Avoid alcohol before the injection, as it can affect B12 absorption

10.1002/cbdv.201900094

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Aminoacyl-tRNA synthetases: potential markers of genetic code development

PaSTRy Solutions of EV-F3 and additive at either 100 or 1000 molar excesses (corresponding to 0.1 mM or 1 mM final concentration) were aliquoted into a 96-well PCR plate containing SYTO9 dye (Thermo Fisher Scientific) in triplicate with 50 mM HEPES, pH 8.0, 200 mM NaCl buffer, before centrifugation for 3 min at 4 C

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