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J Hosp Pharm

10.1038/s41467-021-27837-w 122 Van VlietE

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To keep Hb working efficiently, it must remain in a reduced state, as, if the Fe 2+ within its prosthetic group is oxidized to ferric (Fe +3 ) heme, it forms methemoglobin (metHb), which dramatically decreases the Hbs oxygen affinity [4]

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After 48 h, the viral supernatants were collected and filtered through a 0.45 m membrane filter