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Protein degradation via the proteasome is a net consumer of ATP, although the amount as a proportion of the total cellular energy usage is thought to be relatively small [10]

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Until the recent breakthrough on preparation of 15 N-labeled cobalamins via biosynthesis [8] and further development of the first multiple stable isotope dilution assay (SIDA) that simultaneously determines the four natural vitamers [7], the quantification of B12 in foods has been relying on methods mainly applying microbiological assays [9, 10], (U)HPLC-UV [11,12,13,14,15,16,17], and occasionally LC-ICP-MS [18]

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10.1161/ATVBAHA.117.309575 201 Van DeventerH

We compared the plasma metabolomes between WT-d7 and WT-d119 rats, a time interval during which diabetic changes progressed (Fig