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10 The QM/MM simulations employing the density functional theory (DFT) QM description of a truncated -cyano acrylamide inhibitor and starting from a deprotonated Cys797 confirmed the Michael reaction mechanism involving an intermediate

doi: 10.1016/j.brainresbull.2016.05.003 54 NelsonBSHildebrandtTWallischP

Excess probes were washed off with 2x saline sodium citrate (SSC) (Cat#15557-044, Invitrogen) and deionised formamide (Cat#AM9342, Ambion), followed by a 30 min incubation with buffer W (#121300313, Nanostring slide preparation kit) to block nonspecific signal

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Products of PPP include NADPH, which is utilized for cytoplasmic integrity