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FCCP (10 M) was used 30 min prior to TMRE staining as a positive control of mitochondrial membrane potential loss

Vion AC, Perovic T, Petit C, Hollfinger I, Bartels-Klein E, Frampton E, Gordon E, Claesson-Welsh L, Gerhardt H (2021) Endothelial cell orientation and polarity are controlled by shear stress and VEGF through distinct signaling pathways

In experiments with primary patient-derived cells, responses were measured after 3 days of incubation

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doi: 10.1093/bib/bbae300 41 BrinkmannUKontermannRE

The samples were injected into an Agilent Eclipse Plus reversed-phase C18 column (2.1 mm 150 mm, 1.8 m particle size, 95 pore size) for separation