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At this point, in each well the buffer was replaced with 600 L uptake buffer containing compound and 0.12 Ci (80-110 mCi/mmol) of L-[3,3- 14 C]-cystine (Perkin Elmer) and incubated for 3 minutes at 37 C

However, in the context of mitochondrial dysfunction, the balance between ROS production and antioxidant defense mechanisms is disrupted, leading to an excessive accumulation of ROS [10]

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Shrestha AB, Pokharel P, Sapkota UH, et al

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The role of Zn in the development of ROP is controversial and not well studied but could affect the development of ROP by several mechanisms: (1) as a potent antioxidant