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Another aliquot of the bottom phase was mixed with an acetonitrile/isopropanol (1:1, v/v) mixture, evaporated, then resuspended in 5% methanol with 0.2% formic acid, again including CUDA and Val-Tyr-Val as internal standards

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doi: 10.1182/bloodadvances.2020001742 15 HopeKJJinLDickJE

In fact, such studies revealed that 4-HNE adduction resulted in rapid disappearance of microtubule networks 84 , tubulin crosslinking and inhibition of polymerisation 74, 85 , as well as the spontaneous generation of tubulin dimers 81

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Nevertheless, we believe that the extracts from these raw vegetables and fruits may also work better via injection than ingestion for their anticancer activities